Publications - Kessenbrock Lab

Featured Pubs

A novel role for matrix metalloproteinases in regulating mammary stem cell function via the Wnt signaling pathway.

Kessenbrock et al, Cell Stem Cell, 2013

Matrix metalloproteinases as regulators of the tumor microenvironment

Kessenbrock et al, Cell, 2010

Profiling human breast epithelial cells using single cell RNA sequencing reveals spectrum of cell diversity

Nguyen & Pervolarakis et al, Nat. Commun. 2018

Defining immunosuppressive myeloid cell populations in the spleen of tumor bearing mice in single cell transcriptional space.

Alshetaiwi et al, Science Immunology, 2020

PUBLICATION LIST

GOOGLE SCHOLAR: LINK

Peer-Reviewed:

2020

Pervolarakis N, Nguyen QH, Williams J, Gong Y, Gutierrez G, Sun P, Jhutty D, Zheng GX, Nemec CM, Dai X, Watanabe K^#, Kessenbrock K^#. Integrated single-cell transcriptomics and chromatin accessibility analysis reveals novel regulators of mammary epithelial cell identity. Cell Reports. 2020 Oct 20; 33(3):108273. LINK

Alshetaiwi H, Pervolarakis N, McIntyre LL, Ma D, Nguyen QH, Rath JA, Nee K, Hernandez G, Evans K, Torosian L, Silva A, Walsh C, Kessenbrock K. Defining the emergence of myeloid-derived suppressor cells in breast cancer using single-cell transcriptomics. Science Immunology. 2020 Feb 21;5(44). pii: eaay6017. doi: 10.1126/sciimmunol.aay6017

Haensel DH, Jin S, Cinco R, Sun P, Nguyen QH, Cang Z, Dragan M, Gong Y, MacLean AL, Kessenbrock K, Gratton E, Nie Q, Dai X. Defining epidermal basal cell states during skin homeostasis and wound healing using single-cell transcriptomics. Cell Reports. 2020 Mar 17;30(11):3932-3947.e6. doi: 10.1016/j.celrep.2020.02.091.

Sun P, Dragan M, Vu R, Haensel D, Gutierrez G, Nguyen QH, Ma X, Huang DH, Watanabe K, Greenberg E, Wu J, Atwood S, Pearlman E, Han W, Kessenbrock K, and Dai X. Epidermis-intrinsic transcriptional mechanism coordinates barrier maintenance and neutrophil accumulation during skin inflammation. The Journal of Investigative Dermatology. In press

2019

Samocha A, Doh H, Kessenbrock K and Roose JP. Unraveling Heterogeneity in Epithelial Cell Fates of the Mammary Gland and Breast Cancer. Cancers 2019, 11(10), 1423; https://doi.org/10.3390/cancers11101423

Chen W, Morabito S, Kessenbrock K, Enver T, Meyer KB, Teschendorff AE. Single-cell landscape in mammary epithelium reveals bipotent-like cells associated with breast cancer risk and outcome. Communications Biology. volume 2, Article number: 306 (2019). https://www.nature.com/articles/s42003-019-0554-8

Guerrero-Juarez CF, Dedhia PH, Jin S, Ruiz-Vega R, Ma D, Liu Y, Yamaga K, Shestova O, Gay DL, Yang Z, Kessenbrock K, Nie Q, Pear WS, Cotsarelis G, Plikus MV. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds. Nature Communications. 2019 Feb 8;10(1):650. doi: 10.1038/s41467-018-08247-x.

2018

Lawson DA^#, Kessenbrock K^#, Davis RT, Pervolarakis N, Werb Z. Tumour Heterogeneity and metastasis in single-cell resolution. Nature Cell Biology. 2018 20, 1349-1360. https://www.nature.com/articles/s41556-018-0236-7 ^#corresponding author

Nguyen QH*, Pervolarakis N*, Blake K, Ma D, Davis RT, James J, Phung AT, Willey E, Kumar R, Jabart E, Driver I, Rock J, Goga A, Khan SA, Lawson DA, Werb Z^#, Kessenbrock K^#. Profiling human breast epithelial cells using single cell RNA sequencing reveals spectrum of cell diversity. Nature Communications 2018 May 23;9(1):2028. https://rdcu.be/OZmF ^#corresponding author; *Co-first author

Nguyen QH, Pervolarakis N, Nee K, Kessenbrock K^#. Experimental considerations for single cell RNA sequencing approaches. Front. Cell. Dev. Biol. 2018 Sep 4;6:108. https://doi.org/10.3389/fcell.2018.00108 ^#corresponding author

Qiu X, Lombardo JA , Westerhof TM , Pennell M , Ng A , Alshetaiwi H , Luna BM , Nelson EL , Kessenbrock K , Hui EE , Haun JB .Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells. Lab Chip. 2018 18:2776-2786. doi:10.1039/c8lc00507a.

Takai K, Drain AP, Lawson DA, Littlepage LE, Karpuj M, Kessenbrock K, Le A, Inoue K, Weaver VM and Werb Z. Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive, basal-like breast cancers. Genes & Dev. (2018).

2017

Kessenbrock K*, Smith P*, Steenbeek S, Pervolarakis N, Kumar R, Minami Y, Goga A, Hinck L, Werb Z. Diverse regulation of mammary epithelial growth and branching morphogenesis through non-canonical Wnt signaling. Proc. Natl. Acad. Sci. 2017. 114(12):3121-3126 *contributed equally

2016

Horiuchi D, Zhou AY, Corella AN, Yau C, Balakrishnan S, Kessenbrock K, Lawson DA, Anderton BN, Bazarov AV, Eyob H, Yaswen P, McManus MT, Rugo HS, Werb Z, Goga A. PIM kinase inhibition presents a novel targeted therapy against triple-negative breast tumors with elevated MYC expression. Nat Med. 2016 Nov;22(11):1321-1329. doi: 10.1038/nm.4213.

Park J, Wysocki RW, Amoozgar Z, Maiorino L, Fein MR, Jorns J, Schott AF, Kinugasa-Katayama Y, Lee Y, Won NH, Nakasone ES, Hearn SA, Küttner V, Qiu J, Almeida AS, Perurena N, Kessenbrock K, Goldberg MS, Egeblad M. Cancer cells induce metastasis-supporting neutrophil extracellular DNA traps. Science Translational Medicine. 2016 Oct 19;8(361):361ra138.

Selected Publications from before Lab Start in 2016

Lawson D.A., Bhakta N., Kessenbrock K., Prummel K., Yu Y., Takai K., Yaswen P., Welm A., Goga A., Werb Z., Single-cell analysis reveals a stem cell transcription program in human metastatic breast cancer cells. Nature. 2015 526:131-5

Kessenbrock K.*, Dijkgraaf G.J.*, Lawson D.A., Littlepage L.E., Shahi P., Pieper U., Werb Z. A role for matrix metalloproteinases in the regulation of mammary stem cells via the Wnt signaling pathway. Cell Stem Cell. 2013; 13:300-13; *equal contribution

Caudrillier A., Kessenbrock K., Gilliss, B.M., Ngyen J.X., Marques M.B., Monestier M., Toy P., Werb Z., Looney M.R., Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury. J Clin Invest. 2012; 122:2661-71.

Kessenbrock K., Plaks V., Werb Z., Matrix Metalloproteinases: Regulators of the Tumor Microenvironment. Cell. 2010; 141:52-67. Review

Kessenbrock K.#, Krumbholz M., Schoenermarck U., Back W., Gross W.L., Werb Z., Groene H.J., Brinkmann V., Jenne D.E. #, Netting neutrophils in autoimmune small-vessel vasculitis. Nat Med. 2009; 15:623-625. #corresponding author

Kessenbrock K.#, Fröhlich L., Sixt M., Lämmermann T., Bateman A., Belaaouaj A., Ring J., Ollerrt M., Fässler R., Jenne D.E. #, Proteinase 3 and neutrophil elastase enhance inflammation by inactivating anti-inflammatory progranulin. J Clin Invest. 2008; 118:2438-2447. #corresponding author

Riedl J.*, Crevenna A.H.*, Kessenbrock K., Yu J.H., Neukirchen D., Bista M., Bradke F., Jenne D.E., Holak T.A., Werb Z., Sixt M., Wedlich-Soldner R., Lifeact – a novel versatile marker for the visualization of F-actin. Nat Meth. 2008; 5:605-607. *equal contribution

Kessenbrock K., Fritzler M.J., Groves M., Eissfeller P., von Mühlen C.A., Höpfl P., Mahler M., Diverse humoral autoimmunity to the ribosomal P proteins in systemic lupus erythematosus and hepatitis C virus infection. J Mol Med. 2007 85:953-959.

RESEARCH GOALS

The Kessenbrock Lab studies cellular communication in single cell resolution in the context of normal tissue homeostasis and in breast cancer. We want to understand how stem cell behavior is extrinsically regulated by the microenvironment of the mammary stem cell niche and learn how the molecular composition of the niche changes during tumorigenesis. Our interdisciplinary research approach will identify biomarkers for early detection and may ultimately lead to the discovery of novel therapeutic approaches to treat or even prevent breast cancer before it develops.